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yhcN [2019-06-07 09:40:35]
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yhcN [2019-06-07 09:40:35]

spore lipoprotein
locus
BSU09150
pI
5.36
mw
20.84 kDa
protein length
189 aa Sequence Blast
gene length
570 bp Sequence Blast
function
efficient spore [SW|germination]
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    989,022 → 989,591

    Phenotypes of a mutant

  • reduced rate of spore [SW|germination] in L-alanine [pubmed|28333204]

    • The protein

    [SW|Localization]

  • forespore inner membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|BA0E4135244D214B435836F07F608818A31B7E52|yhcN]
    description
    [Pubmed|9611260]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|9611260], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|9611260,15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulation

  • expressed during sporulation ([protein|search|SigF], [protein|search|SigG]) [Pubmed|9611260]
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    additional information

  • the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

    • Biological materials

    Mutant

  • MGNA-A671 (yhcN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/671 NBRP B. subtilis, Japan]
  • 1A924 ( ''yhcN''::''erm''), [Pubmed|12813063], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A924&Search=1A924 BGSC]
  • BKE09150 (Δ[gene|BA0E4135244D214B435836F07F608818A31B7E52|yhcN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAATTCCTCCTTTAT, downstream forward: _UP4_TAAATGAAAGAAGCCGCACA
  • BKK09150 (Δ[gene|BA0E4135244D214B435836F07F608818A31B7E52|yhcN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAATTCCTCCTTTAT, downstream forward: _UP4_TAAATGAAAGAAGCCGCACA

    • References

  • 16497325,9611260,26731423,28333204,30782632